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Molecular biology --- Proteins --- Protéines --- Periodicals. --- Périodiques --- Proteins. --- Chemistry --- Health Sciences --- Biochemistry --- Physiology --- moleculaire biologie --- Animal Biochemistry

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The Handbook of Cell Signaling is a comprehensive work covering all aspects of intracellular signal processing, including extra/intracellular membrane receptors, signal transduction, gene expression/translation, and cellular/organotypic signal responses. The subject matter has been divided into five main parts (each of which is headed by a recognized expert in the field):* Initiation: Extracellular and Membrane Events* Transmission: Effectors and Cytosolic Events * Nuclear Responses: Gene Expression and Translation* Events in Intracellular Compartment

Histology. Cytology --- General biochemistry --- Cell interaction. --- Cellular signal transduction. --- Cellular signal transduction. Cell interaction. --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry

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Volume 323 of Methods in Enzymology is dedicated to the energetics of biological macromolecules. Understanding the molecular mechanisms underlying a biological process requires detailed knowledge of the structural relationships within the system and an equally detailed understanding of the energetic driving forces that control the structural interactions. This volume presents modern thermodynamic techniques currently being utilized to study the energetic driving forces in biological systems. It will be a useful reference source and textbook for scientists and students whose goal

Bio-energetica --- Bioenergetics --- Biological molecules --- Biologische moleculen --- Biomoleculen --- Biomolecules --- Biomolécules --- Bioénergétique --- Dynamica [Thermo] --- Dynamique [Thermo] --- Energetica [Bio-] --- Energie biologique --- Energy [Biological ] --- Energy balance (Biology) --- Energy budget (Biology) --- Energy dynamics (Ecology) --- Energy utilization (Biology) --- Macromoleculen --- Macromolecules --- Macromolécules --- Moleculen [Bio] --- Moleculen [Biologische ] --- Moleculen [Macro] --- Molecules [Biological ] --- Molécules [Macro] --- Molécules biologiques --- Thermodynamica --- Thermodynamics --- Thermodynamique --- Allosteric regulation. --- Bioenergetics. --- Energy Metabolism. --- Macromolecular Systems. --- Macromolecules. --- Molecular Conformation. --- Thermodynamics. --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Chemistry, Physical and theoretical --- Dynamics --- Mechanics --- Physics --- Heat --- Heat-engines --- Quantum theory --- Biochemistry --- Energy budget (Geophysics) --- Molecules --- Supramolecular chemistry --- Enzymology --- Macromolecular Systems --- Allosteric Regulation. --- Macromolécules --- Bioénergétique --- Molecular biology

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Capillary electrophoresis (CE) is one of the premier methods for the rapid analysis of pharmaceutical proteins and peptides, and thus now even more important with the sequencing of the human genome and the subsequent advent of proteomics. In Capillary Electrophoresis of Proteins and Peptides, leading academic scientists and expert laboratorians working in the pharmaceutical industry describe their best CE techniques in step-by-step detail for easy reproduction. The authors present cutting-edge techniques for both capillary coatings and analytical detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Additional chapters discuss CE and capillary isoelectric focusing, combined with electrospray mass spectrometry detection, for performing proteomic studies. Areas of special interest covered include proteomic applications, as well as applications in the field of protein-ligand binding. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Capillary Electrophoresis of Proteins and Peptides will prove essential to both new and experienced investigators carrying out significant protein and peptide studies in biotechnology, drug discovery, and pharmaceutical research and development.

Capillary electrophoresis --- Proteins --- Peptides --- Electrophoresis, Capillary --- Separation --- methods --- analysis --- Capillary electrophoresis -- Laboratory manuals. --- Peptides -- Separation -- Laboratory manuals. --- Proteins -- Separation -- Laboratory manuals. --- Electrophoresis --- Amino Acids, Peptides, and Proteins --- Investigative Techniques --- Chemistry Techniques, Analytical --- Chemicals and Drugs --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Electrochemical Techniques --- Methods --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Organic compounds --- Amino acids --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Capillary gel electrophoresis --- Capillary zone electrophoresis --- Gel electrophoresis --- Zone electrophoresis --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Capillary electrophoresis - Laboratory manuals --- Proteins - Separation - Laboratory manuals --- Peptides - Separation - Laboratory manuals --- Electrophoresis, Capillary - methods --- Proteins - analysis

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High performance liquid chromatography (HPLC) plays a critical role today in both our understanding of biological processes and in the development of peptide and protein-based pharmaceuticals. In HPLC of Peptides and Proteins: Methods and Protocols, leading experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology. These applications include proteolytic mapping, posttranslational modification, neuropeptide processing, glycopeptides and glycoproteins, MHC-binding peptides, toxins/venoms, membrane proteins, antibodies, combinatorial and proteome analysis, and enzymatic activity. Each readily reproducible protocol contains background notes, step-by-step instructions, reagent and equipment lists, and tips on both troubleshooting and avoiding known pitfalls. Comprehensive and easy-to-follow, HPLC of Peptides and Proteins: Methods and Protocols offers every biologist and biotechnologist at all levels of expertise ready access to the powerful tools needed to develop and carry out peptide and protein separations and analyses today.

Electronic books. -- local. --- High performance liquid chromatography. --- Peptides -- Analysis. --- Proteins -- Analysis. --- Peptides --- Proteins --- High performance liquid chromatography --- Chromatography, High Pressure Liquid --- Chemistry --- Methods --- Amino Acids, Peptides, and Proteins --- Chromatography, Liquid --- Investigative Techniques --- Natural Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemicals and Drugs --- Disciplines and Occupations --- Chromatography --- Chemistry Techniques, Analytical --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Analysis --- Analysis. --- High performance chromatography --- High pressure liquid chromatography --- High sensitivity liquid chromatography --- High speed liquid chromatography --- HPLC (Chromatography) --- High pressure (Science) --- Liquid chromatography --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Medical sciences --- Composition